Table 1.
Bacteria/bacteriophage/ plasmid | Relevant characteristics | Source or reference |
---|---|---|
Bacteria | ||
197N | parental B. avium strain; NalR | Temple et al. (1998) |
PAS78 | wlbL insertion mutant of B. avium 197N; wlbL::Tn5; KanR, NalR | This study |
PAS85 | wlbA insertion mutant of B. avium 197N; wlbA::Tn5; KanR, NalR | This study |
E. coli S17.1(γpir) | SmR | Simon et al. (1983) |
E. coli DH5α | Life Technologies | |
Bacteriophage | ||
Ba1c1 | clear plaque derivative of B. avium phage Ba1 | Shelton et al. (submitted) |
Plasmids | ||
pGEM-11z(f) | E. coli cloning vector; ApR | Promega |
pGEM-av | NotI 15 kb wlb locus of B. avium in pGEM-11zf; ApR | This study |
pLAFR5 | broad host range vector; TcR | Keen et al. (1988) |
pLAF-av |
NotI 15 kb wlb locus of B. avium from pGEM-av cloned into pLAFR5; TcR |
This study |
pLAF-pe | wlb locus of B. pertussis in pLAFR5; TcR | Allen et al. (1998) |
pLAF-br | wlb locus of B. bronchiseptica in pLAFR5; TcR | Allen et al. (1998) |
pLAF-pa | wlb locus of B. parapertussis in pLAFR5; TcR | Allen et al. (1998) |