Table 1.
Monoclonal Antibodies Recognizing Surface Antigens on Subsets of Mouse Liver Cells
| Antibody | Isotype | Classification | Percent of CD45- Liver NPCs Positive |
|
|---|---|---|---|---|
| Untreated | DDC-treated | |||
| MIC1-1C3 | rat IgM | Ductal | 8–24 | 19–60 |
| OC2-1C6 | rat IgG1 | Periductal | <1 | 4–30 |
| OC2-1D11 | rat IgG1 | Ductal | 0–10 | 14–60 |
| OC2-2A6 | rat IgG1 | Periductal | <1 | 2–29 |
| OC2-2F3 | rat IgG1 | Ductal | <1 | 1–21 |
| OC2-3C5 | rat IgG1 | Periductal + | 5–22 | 21–58 |
| OC2-3C7 | rat IgG1 | Periductal | 1–7 | 2–10 |
| OC2-4E8 | rat IgG1 | Periductal | 0–10 | 22–48 |
| OC2-6E10 | rat IgG1 | Periductal | <1 | 1–11 |
Antibody labeling patterns are classified as ductal or periductal based on specificities observed on liver tissue sections. The ranges of cell labeling frequencies are based on the evaluation of four CD45- NPC preparations by flow cytometry.