Table 2.

Molecular Characterization of Gene Expression in Sorted Subpopulations of Normal and DDC-Treated Liver Cells

			CK7	CK19	Albumin	Desmin	vWF	AFP
DDC-treated CD45- NPC			1	1	1	1	1	1
Untreated CD45- NPC			↓ 0.26	= 0.58	= 0.72	↓ 0.38	↓ 0.44	↓ 0.40
Hepatocytes			↓ 0.06	↓ 0.13	↑ 15.1	= 0.85	= 1.12	↑ 16.1
Untreated CD45- NPC	MIC1-1C3	+	↑ 6.93	↑ 15.5	= 0.64	↓ <0.01	↓ 0.47	↑ 2.76
		−	↓ 0.39	= 0.81	↓ 0.28	= 1.16	↓ 0.34	↓ 0.14
DDC treated CD45- NPC	MIC1-1C3	+	= 1.02	= 1.34	= 0.53	↓ 0.17	↓ 0.07	↑ 3.25
		−	= 0.55	= 0.77	= 1.18	= 1.62	= 1.93	↓ 0.46
	OC2-1C6	+	↓ 0.12	↓ 0.21	↓ 0.26	↑ 3.59	↓ 0.11	↓ 0.31
		−	= 0.67	= 0.81	= 0.85	= 1.06	↓ 0.44	= 1.74
	OC2-1D11	+	= 0.70	= 0.65	↓ 0.34	= 0.84	↓ 0.38	= 1.18
		−	↓ 0.28	↓ 0.24	↓ 0.19	↑ 2.31	↑ 2.48	↓ 0.19
	OC2-2A6	+	↑ 2.67	↑ 15.4	↓ 0.13	↓ 0.18	↓ 0.17	↓ <0.01
		−	↑ 3.74	↑ 2.42	= 1.15	↑ 3.62	↑ 2.28	= 1.24
	OC2-2F3	+	= 1.40	↑ 3.69	= 0.54	↓ 0.43	↓ 0.20	↓ 0.46
		−	= 1.49	= 1.05	↓ 0.35	= 1.16	= 1.80	↓ <0.01
	OC2-3C5	+	↓ 0.22	= 0.60	= 0.65	= 1.70	= 0.52	↓ 0.45
		−	= 1.50	= 1.83	= 0.62	= 1.24	= 0.86	= 1.18
	OC2-3C7	+	↓ 0.20	↓ 0.41	= 0.71	= 0.66	↓ 0.13	↑ 2.22
		−	↓ 0.39	= 0.84	= 0.92	↑ 2.09	↓ 0.31	↓ 0.47
	OC2-4E8	+	↓ 0.23	↓ 0.25	↓ 0.24	↓ 0.28	↓ 0.14	= 0.94
		−	= 0.54	= 0.87	↓ 0.36	= 0.51	= 0.50	= 0.52
	OC2-6E10	+	= 1.21	↑ 3.13	↓ 0.28	= 0.74	↓ 0.11	= 1.36
		−	= 1.10	= 1.59	= 0.68	= 1.53	↑ 2.41	= 0.96

Complementary DNA was obtained from cells isolated from DDC-treated liver tissue by FACS (50,000 cells per sort) and assessed by QPCR for the expression of representative genes. Individual ΔCt results were normalized to GAPDH and are reported relative to the levels observed in unsorted NPC from DDC-treated liver. The normalized ΔCt results used were the averages of three independent cDNA amplifications.