Figure 4.

Purification and quantification of the selected membrane protein target. (A) Analytical gel filtration from medium-scale shake flask cultivations using the target protein, EM03. The chromatogram shows values for an equal OD₆₀₀ -value of cells in the different samples, normalized with respect to the highest top peak absorbance set equal to one. (B) Estimated peak areas of analytical gel filtration chromatograms from the production of the target EM03. The values show calculated peak areas for an equal OD₆₀₀ -value of cells in the different samples, normalized with respect to the largest top peak area set equal to one.