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. 2011 May 15;11:13. doi: 10.1186/1475-2867-11-13

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Copyright ©2011 Patrikidou et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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NFκB activation in PC cell lines. (A) Nuclear and cytoplasmic NFκB levels in PC-3 (left) and LnCaP cells (middle). Right: Nuclear extracts of PC-3 cells at baseline and following bortezomib incubation (1 μM for 60 min). (B) NFκB EMSA. Lane 1: negative control; Lane 2: PC-3 cells; Lane 3: LnCaP cells; Lane 4: HeLa cells (6 hrs post-infection). (C) Total cellular IκBα levels in PC-3 (left) and LnCaP cells (right). (D) Total cellular IκBα levels in PC-3 cells following serial incubations with bortezomib 1 μM.