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Proteasome activity in PC cell lines. 20S proteasome activity (RFU/μg) in PC-3 (left; 208.58, SD: 3.24) and LnCaP cells (right; 45.2, SD: 0.2). All measurements in quintuplicate (p < 0.001). Small graph: % inhibition of baseline 20S proteasome inhibition produced by bortezomib incubations (1 μM for 60 minutes) in LnCaP (left) and PC-3 cells (middle); % activity inhibition of control 20S proteasome produced by lactacystin (right). All measurements in triplicate (p < 0.001).
