. 2011 May 16;11:85. doi: 10.1186/1471-2229-11-85

Table 2.

The expression of RBCS1 isoforms in leaves of different coffee genotypes.

Genotype	Cultivar	Origin	Trial	CaCc/CaCe
C. canephora

	`L21`		`I`	`65.93`
	`14 ^T`	`Conilon`	`G`	`1324.28`
	`22 ^S`	`Conilon`	`G`	`247,10`
	`73 ^T`	`Conilon`	`G`	`260.65`
	`120 ^T`	`Conilon`	`G`	`236.71`

C. arabica ("pure")

	`Rubi ^S`	`Mundo Novo x Catuaí`	`E`	`0.00013`
	`Bourbon`		`I`	`0.00014`
	`Typica`		`I`	`0.00017`
	`Catuaí`	`Mundo Novo x Caturra`	`I`	`0.00021`

C. arabica ("introgressed")

	`HT832/1`	`Timor hybrid`	`E`	`0.00004`
	`HT832/2`	`Timor hybrid`	`I`	`0.40102`
	`Icatú`	`C. canephora x Bourbon`	`I`	`9.33`
	`IAPAR59 ^T`	`Villa Sarchi x HT832/2 (Sarchimor)`	`I`	`3.22`
	`Tupi`	`Villa Sarchi x HT832/2 (Sarchimor)`	`I`	`2.63`
	`Obabã`	`[Villa Sarchi x HT832/2] x Catuaí`	`I`	`1.26`
	`IPR97`	`Sarchimor`	`I`	`4.98`
	`IPR98`	`Sarchimor`	`I`	`21.65`
	`IPR102`	`Icatú x Catuaí`	`I`	`0.00427`
	`IPR106`	`Icatú x Catuaí`	`E`	`0.03212`
	`IPR107`	`Sarchimor x Mundo Novo`	`E`	`0.12255`

C. eugenioides			`I`	`0.00035`

Expression was measured by the ratio CaCc/CaCe where CaCc (RBCS1-Cc) and CaCe (RBCS1-Ce) values were obtained using the C18244 and E18244 primer pairs (Table 1), respectively. Relative quantifications (RQ) were normalised using the expression of the CcUBQ10 (in the case of C. canephora) or GAPDH (for other species) reference genes. The CaCc/CaCe ratio corresponded to (1+E)^-ΔCt, where ΔCt = Ct_meanCaCc - Ct_meanCaCe with E as the efficiency of the gene amplification. Leaves were collected from plants grown in the field at the Embrapa Cerrados (E), IAPAR station (I) and UFV greenhouse (G). When known, the reaction to drought is indicated (^T= Tolerant and ^S= Susceptible). All Sarchimors are derived from HT832/2.