Figure 9. Effects of UV90 mutation on expression of FRQ protein.

Both strains carry the ras-1^bd, csp-1 and chol-1 mutations and were grown on high choline to repair the chol-1 defect. Cultures were grown in DD for varying lengths of time and protein extracts were assayed for FRQ protein by immunoblotting. A. Relative level of FRQ, normalized to total protein by Coomassie staining. Solid circles: UV90 wild-type; open circles: UV90 mutant. Points are from a single series of experiments; similar results were obtained in a replicate experiment. B. Upper panel: Immunoblotting of FRQ protein for one cycle, 3–27 h in DD, corresponding to the time-series in A. UV90 wild-type samples are on the left half and UV90 mutant samples on the right half of the blot. Lower panel: Coomassie stained membrane of samples in upper panel. C. Ratios of relative FRQ levels in UV90 vs. UV90⁺. Ratios were calculated at three peak time points (21, 42 and 63 hours in DD) in three independent experiments and mean ratios are plotted ± one S.E.M. *: significantly less than 1.00, p<0.05. **: significantly less than 1.00, p<0.01.