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. Author manuscript; available in PMC: 2012 May 5.
Published in final edited form as: DNA Repair (Amst). 2011 Mar 22;10(5):497–505. doi: 10.1016/j.dnarep.2011.02.003

Figure 2. The high fidelity of WT replicative polymerases is not maintained in dinucleotide microsatellite sequences.

Figure 2

Gap-filling DNA synthesis reactions were performed using either (A). Polδ WT, or (B). Polε WT and the pSStu2/pSAStu2 complementary DNA substrates. The Pol MFest was calculated from the HSV-tk mutant frequency, using the data presented in Table 2: Pol MFest = [(HSV-tk MF of the indicated polymerase reaction) – (unfilled gap MF)] for each region. Graphs compare the Pol MFest within the HSV-tk coding sequence (Open bars) to that within the [GT]10 or [CA]10 microsatellite sequences (solid bars) for each substrate.