Table 1. Observed HSV-tk mutant frequencies resulting from in vitro replicative polymerase DNA synthesis.
| Polymerase | HSV-tk mutant frequency ×10-4 (± std. dev) a | |||
|---|---|---|---|---|
| pSStu2 | pSAStu2 | |||
| [GT]10 substrate | N b | [CA]10 substrate | N | |
| None | ||||
| ssDNAc | 3.8 | 1.5 | ||
| Unfilled Gapd | 4.6 ± 0.6 (4) | 44 | 12.0 ± 3 (4) | 40 |
| Polδ | ||||
| WT | 43.0 ± 13 (5) | 96 | 21.0 ± 6 (5) | 90 |
| Exo- | 69.0 ± 18 (5) | 99 | 46.0 ± 23 (3) | 94 |
| Polε | ||||
| WT | 20 ± 3 (4) | 95 | 31.0 ± 6 (3) | 61 |
| Exo- | 42 (1) | 39 | 65 (2) | 93 |
a
Number of independent determinations is given in parentheses
b
Number of independent mutants sequenced
c
Electroporation of ssDNA used to create the gapped substrates
d
Electroporation of unfilled gapped DNA substrate