Table 4. Comparison of wild-type polymerase fidelity for different types of misalignment-based errors.
| Substrate/ Mutational event |
Pol MFesta × 10-4 (number observed) | |
|---|---|---|
| Pol δ WT | Pol ε WT | |
| pSStu2 [GT]10 | ||
| Microsatellite Indelsb | 25 (62) | 9.0 (53) |
| Coding Indelc | 1.1 (3) | 0.16 (1) |
| Coding Indel/sited | 0.048 | 0.007 |
| Fold differencee | 500 | 1300 |
| pSAStu2 [CA]10 | ||
| Microsatellite Indels | 11 (79) | 17 (49) |
| Coding Indel | ≤ 0.06 (0) | ≤ 0.39 (0) |
| Coding Indel/site | ≤ 0.003 | ≤0.02 |
| Fold difference | ≥ 3600 | ≥ 800 |
a
Pol MFest values were calculated for each error from the data presented in Tables 2 and 3. Pol MFest = [HSV-tk MF for the indicated reaction – unfilled gap MF] × Proportion of total
b
Frequency of unit-based indel errors within the indicated microsatellite
c
Frequency of indel errors at 2-3 unit repeated sequences within the indicated HSV-tk target sequence.
d
Frequency of indel errors/ site. There are 23 sites of 2-3 unit repeated sequences within the MluI-StuI mutational target (Figure S1).
e
Microsatellite indel frequency / Coding indel frequency per site