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. 2011 Jun 23;7(6):e1002152. doi: 10.1371/journal.pgen.1002152

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A. Translesion synthesis by E. coli Klenow fragment (2,5 nM) and Pol I (20 nM) on 34-mer templates harbouring a single lesion (X) at position 16. The polymerases have to add two nucleotides before encountering the lesion. oG = 8-oxoguanine; dHT = di-hydrothymidine; Tg = thymine-glycol and THF = tetra-hydrofurane, an abasic site analogue. The concentrations used for the two DNA polymerases were chosen for yielding the same activity on undamaged DNA. B. Nucleotide selection. Extension reactions directly on a lesion were carried out for 5 min in the presence of a single dNTP (0.1 mM) as indicated for each lane.