Figure 2. Summary of genome-wide distribution of 5-hmC in human H1 ES cells.

A. Chromosome-wide distributions of 5-hmC-enriched and -unenriched input genomic DNA reads (reads/million), compared to the distribution expected by chance if reads were randomly distributed amongst chromosomes (10⁶/hg18 length X chromosome length, with expected values divided by 2 for chromosome X). B. Association between 82,221 5-hmC enriched regions (p-value threshold of 1e-8) and annotated genomic features (obtained through UCSC Tables, NCBI36/hg18 and [15]). Values are represented as the fold change in percentage of peaks overlapping a defined feature over the percent expected by chance give the genomic base coverage of that feature. Shown to the right is the fraction of total peaks corresponding to each genomic feature for reference. C. Observed-to-Expected (O/E) ratios of all possible dinucleotides in 5-hmC-enriched regions (n = 82,221, p-value threshold of 1e-8), CpG Islands (NCBI36/hg18, n = 28,226), and randomly selected genomic regions (n>100,000). D. The cumulative fraction of genomic regions with a given GC content plotted for 5-hmC-enriched regions (n = 82,221, p-value threshold of 1e-8), CpG Islands (NCBI36/hg18, n = 28,226), and randomly selected genomic regions (n>100,000).