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. 2010 Apr 15;12(8):1009–1059. doi: 10.1089/ars.2009.2597

FIG. 13.

FIG. 13.

Effects of oxidized phospholipids on endothelial barrier function. (A) Bi-phasic concentration-dependent effects of OxPAPC on endothelial barrier function. Transendothelial electrical resistance (TER) reflecting barrier properties of EC monolayer was recorded in pulmonary ECs exposed to OxPAPC. OxPAPC exhibited prominent barrier-protective effects at concentrations below 20 μg/ml. Higher OxPAPC concentrations (50 and 100 μg/ml) caused barrier-disruptive response. (B) Nonfragmented, but not oxidatively fragmented PLs exhibit barrier-protective effect. TER was measured in pulmonary EC monolayers exposed to HPLC-purified isoprostanes esterified in PC (upper panel), or fragmented product PGPC (lower panel). Another fragmented product POVPC exhibited disruptive effects similar to that shown for PGPC. Note that individual components of OxPAPC, such as PEIPC and PGPC, demonstrate opposite action on EC barrier. Adapted from (28). (C) OxPAPC attenuates thrombin-induced elevation of EC permeability. TER was monitored across the confluent EC monolayers treated with OxPAPC (20 μg/ml) and thrombin (0.5 U/ml) added at the times shown by arrows. Adapted from (31).