Table 4. Sets of primers for PCR and RT-PCR of target sequences.

Amplicon	Forward Primer	Reverse Primer	AmpliconLength	Access Number
	PCR
NFM	5′- TCG-GCG-ATC-TCT-TCT-TTA-GCG- 3′	5′- CGG-AGC-AA-TCA-CGA-AGA-GGA- 3′	254 bp	NM_017029
NFL	5′- TCC-CCC-TTG-AGT-CCT-CTT-GA- 3′	5′- ACT-TGT-CCC-TTC-ACG-GGA-GA- 3′	249 bp	X53981
MBP	5′- CTT-CCG-AAG-GCC-TGA-TGT-GAT-3′	5′- TAA-AGA-AGC-GCC-CGA-TGG-A-3′	158 bp	K00512
GFAP	5′- TCC-AGC-CCG-TCC-CTC-AAT-AA-3′	5′- TCC-CGA-AGT-TCT-GCC-TGG-TAA-3′	418 bp	Z48978
MPZ5′	5′- CTT-GCC-CCT-ACC-CCA-GCT-AT- 3′	5′- TCT-CCT-TGG-CTG-GCT-CTC-AAT- 3′	184 bp	NM_017027
MPZ3′	5′-CCC-TGG-CCA-TTG-TGG-TTT-ACA-3′	5′-TGG-ATG-CGC-TCC-TTG-AAG-GT-3′	291 bp	NM_017027
ACT	5′- AAC-ACC-CCA-GCC-ATG-TAC-G- 3′	5′- ATG-TCA-CGC-ACG-ATT-TCC-C- 3′	254 bp	M10277
ALB	5′-GGG-ATT-TAG-TTA-AAC-AAC-TT.-3′	5′- AAA-GGT-TAC-CCA-CTT-CAT-TG- 3′	206 bp	M16825
AFP	5′-ACC-CAT-GCA-TCT-GTG-ACA-TA- 3′	5′-AGT-AAA-ATG-CAT-GTT-GCC-TG- 3′	252 bp	J02816
	RT-PCR
NFM	5′- TCG-GCG-ATC-TCT-TCT-TTA-GCG- 3′	5′- CGG-AGC-AA-TCA-CGA-AGA-GGA- 3′	254 bp	NM_017029
NFL	5′- GAA-GAG-TGG-TTC-AAG-AGC-CGC-3′	5 - TCG-TGC-TTC-GCA-GCT-CAT-TC-3′	256 bp	NM_031783
ACT	5′- AAC-ACC-CCA-GCC-ATG-TAC-G - 3′	5′- ATG-TCA-CGC-ACG-ATT-TCC-C - 3′	254 bp	M10277
AFP	5′-CAG-TGA-GGA-GAA-ACG-GTC-CG- 3′	5′-ATG-GTC-TGT-AGG-GCT-CGG-CC- 3′	252 bp	NM_012493

Genomic DNA amplicons used for the positional mapping of target-gene sequences relative to the NM and amplicons for reverse transcriptase-PCR analysis of mRNA (RT-PCR) used for studying the expression of the corresponding genes. NFM: Neurofilament medium, chromosome location 15p12; NFL: Neurofilament light, chromosome 14; MBP: myelin basic protein, chromosome 18q11–q13; GFAP: Glial fibrillary acidic protein, chromosome 10q32.1; MPZ5′: Myelin protein zero region 5′, chromosome 13q24–q25; MPZ3′: Myelin protein zero region 3′, chromosome 13q24–q25; ACT: β-actin, chromosome 12q11; ALB: albumin, chromosome 14p22; AFP: α-fetoprotein, chromosome 14p21.