Table 1.
Mutation analysis of GTase constructs of hCE
| Mutants | GTP binding |
| GT222-574 | +++ |
| GT229-569 | +++ |
| GT240-569 | insoluble |
| GT224-468 | + |
| GT224-475 | + |
| GT224-551 | +/− |
| GT224-567 | +++ |
| GT229-468 | + |
| GT229-475 | + |
| GT229-551 | + |
| GT229-567 | +++ |
| GT229-567 (M335I/M437V/RM476KV/M543V/M667I) | +++ |
| GT229-569 (M396A/M437A/M527A) | +/− |
| (M335A/M437A/M527A/M543A) | − |
| (M335A/M396A/M437A/M527A/M543A) | − |
| GT229-569 E234A | +++ |
| GT229-569 K458A | − |
| GT229-569 K460A | − |
| GT229-569 R528A | + |
| GT229-569 R530A | − |
| GT229-569 D532A | − |
| GT229-569 K533A | − |
GT229-567, GT229-569, and the listed methionine mutants containing Se-Met had activities comparable to the corresponding unsubstituted proteins. Activity levels: +++ > 85% of WT; + 10–22% of WT; +/- < 10% of WT; - undetectable.