Table 1.
Number of molecules with stable or fluctuating smFRET
| Classification of smFRET traces | |||||
| Sample | Molecule Count and Percentage | ||||
| Protein | T(°C) | n | Stable High | Stable Mid | Fluctuating |
| N-His IκBα | 21 | 207 | 163 (79%) | 15 (7%) | 29 (14%) |
| C-His IκBα | 21 | 202 | 157 (78%) | 5 (2%) | 40 (20%) |
| N-His IκBα | 24 | 213 | 122 (57%) | 34 (16%) | 57 (27%) |
| N-His IκBα | 37 | 226 | 113 (50%) | 36 (16%) | 77 (34%) |
| N-His IκBα + NFκB | 37 | 207 | 176 (85%) | 19 (9%) | 12 (6%) |
| YLTA IκBα | 24 | 215 | 186 (86%) | 12 (6%) | 17 (8%) |
| N-His IκBα + 0.1 M urea | 24 | 221 | 104 (47%) | 26 (12%) | 91 (41%) |
| N-His IκBα + 0.5 M urea | 24 | 200 | 98 (49%) | 25 (12%) | 77 (39%) |
| Number of transitioning molecules | ||||||
| Protein | T(°C) | n | Stable High | Stable Mid | Fluctuating | Transitioning* |
| N-His IκBα | 21 | 210 | 183 (87%) | 5 (2%) | 18 (9%) | 4 (2%) |
| N-His IκBα | 37 | 201 | 109 (54%) | 21 (10%) | 60 (30%) | 11 (6%) |
*Molecules were classified as transitioning if they spent more than 5 s in one state and then transitioned to another state in which they also spent more than 5 s.