Fig. 5.

Quantification of the relative frequencies of preexisting drug-resistance mutations. (A) A pool of drug-naïve RFP-labeled replicons, isolated from stable replicon cells to generate genetic heterogeneity, is transfected into 51 Cured cells in the presence or absence of drug. The resulting foci are counted. (B) In the absence of drug, an average of 5,993 fluorescent replicon foci is observed. An average of 66 (1.1%) of these foci are resistant to 150 nM HCV-796, and 96 (1.5%) are resistant to 2,400 nM A-782759 (n = 2).