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Representative tracings of Isc from differentiated cultures of human airway epithelia. (A) Non-CF epithelia. (B) CF epithelia. Regular pulses are current required to clamp Vt to 5 mV. Sequential additions were 100 μM apical amiloride (Amil), 100 μM apical DIDS, 10 μM apical forskolin plus 100 μM IBMX (F&I), and 100 μM basolateral bumetanide (Bumet).
