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. Author manuscript; available in PMC: 2011 Dec 23.
Published in final edited form as: Oncogene. 2011 Feb 21;30(25):2859–2873. doi: 10.1038/onc.2011.12

Figure 5. Prdm14 alters expression of pluripotency genes and oncogenes in pre-leukemic and leukemic cells.

Figure 5

5a) Heatmap of gene expression in flow-sorted CLP-like cells obtained at four weeks post transplantation from Prdm14-transduced and EV-transduced mice. Each column represents one sample. Genes depicted are relevant in oncogenesis, tumor suppression, pluripotency, signal transduction, or B cell development. 5b) Expression of Pou5f1, Prdm1, Dlk1, Meg3, Mtor, Il7r, Myb, Scin, and Glp is increased in tumors from Prdm14-transduced mice. Sox2 is downregulated. Levels in tumors are compared to both wild-type whole bone marrow and spleen from EV recipients using Mann-Whitney analysis. Error bars represent median with interquartile range (* indicates p<0.05 and ** indicates p<0.01). 5c) Western blot analysis shows increased MYB protein in Prdm14-induced leukemias, which is seen neither in EV controls nor in pre-leukemic cells. C = 8 week old spleen control; Prdm14 Pre-LL = spleen from a Prdm14 recipient that had not developed a tumor.