FIG. 2.

Inhibition of p38 significantly increases the production of LKS⁺ and Lin⁻c-kit⁺Sca1⁻ cells (LKS⁻ cells). LKS⁺ cells were cultured in vitro in the presence of vehicle (0.1% DMSO) or SB (5 μM) for 7 days. The cells harvested from the cultures were numerated and phenotypically analyzed by flow cytometry. (A) Representative flow cytometric analyses. (B) Number of total mononuclear cells (MNCs). (C) Number of Lin⁻ cells. (D) Number of LSK⁻ cells. (E) Number of LSK⁺ cells. The data in (B–D) are presented as mean±SE (n=3 independent cultures) of number of each cell population recovered from the culture with 1,000 input LSK⁺ cells.