Fig. 5.

The coding region of the viperin mRNA is sufficient for its elevated translation in cells depleted of RNase MRP/P. a Viperin expression in HEp-2 and HeLa cells that were subjected to increasing concentrations of IFN-α for 16 h before harvesting was analyzed by immunoblotting. The concentration of IFN-α ranged from 0 to 1,000 U/ml. As a control, γ-tubulin was analyzed in parallel. b HeLa cells were transfected with a pcDNA3.1 construct containing the coding sequence of viperin with or without the 5′ and 3′ UTRs. The cells were co-transfected with a GST-coding plasmid, which served as a transfection control. After 24 h, the cells were treated with the indicated siRNAs. Forty-eight hours after siRNA transfection, cell lysates were analyzed by immunoblotting using antibodies specific for viperin and GST