Figure 2.

Effect of heat stress on the SUMOylation levels of representative Arabidopsis targets. Seven-day-old seedlings were subjected to heat stress as in Figure 1. HS, heat-stressed seedlings. RT, seedlings not exposed to heat stress. Levels of each protein in the RT and HS MS datasets were estimated by the number of spectral counts in the MS precursor scans that were obtained for each target; these numbers were then normalized by the total number of spectral counts for each MS run. (A) SUMOylated transcription and DNA repair components that increase in abundance during heat stress. (B) SUMOylated proteins that decrease in abundance during heat stress. (C) Members of the SUMO conjugation pathway.