Figure 2.

Phenethyl isothiocyanate (PEITC) induces downregulation of Mcl-1, inactivation of Akt, and activation of Jun N-terminal kinase (JNK) in U937 cells. (a) U937 cells were treated without or with the indicated concentrations of PEITC for 3 and 6 h. (b) U937 cells were treated without or with 8 μM PEITC for 0.5, 1, 2, 3, 4, 6, 12, and 24 h. Total cellular extract were prepared and subjected to western blot analysis using antibody against XIAP, Mcl-1, Bcl-2, Bcl-xL, Bax, and Bad. (c) U937 cells were treated without or with the indicated concentrations of PEITC for 3 and 6 h. (d) U937 cells were treated without or with 8 μM PEITC for 0.5, 1, 2, 3, 4, 6, 12, and 24 h. Total cellular extract were prepared and subjected to western blot analysis using antibodies against cell signaling molecules, including phospho-Akt, Akt, phospho-JNK, and JNK. Each lane was loaded with 30 μg protein. Blots were subsequently stripped and reprobed with antibody against β-actin to ensure equivalent loading and transfer. Two additional studies yielded equivalent results