Figure 2.

Nutlin-3 induces apoptosis in TC cells. (a) Survival of TC cells after 96 h of continuous Nutlin-3 treatment as indicated; values are the mean±S.D. of three independent experiments. (b) Apoptosis induction was analysed after Nutlin-3 treatment for 24 h, by fluorescence microscopy on acridine orange-stained TC cells; values are the mean±S.D. of three experiments. (c) Immunoblot analysis showing upregulation of p53, MDM2 and p21 and enhanced cleavage of PARP and caspase-8 in wild-type p53 TC cells 24 h after Nutlin-3 treatment. The data presented are representative of three independent experiments