Figure 3.

Wild-type p53 is necessary for the Nutlin-3 response. (a–c) Downregulation of p53 reduces the apoptotic response after Nutlin-3 treatment in wild-type p53-expressing TC cells. Cells were treated with scrambled siRNA (siRNA scr) or with p53 siRNA (siRNA p53) for 24 h. Values are the mean±S.D. of three experiments; ^#P<0.05; ^*P<0.01; ^**P<0.005. (d) Successful downregulation of p53 using siRNA, decreases the expression of MDM2 and p21, and decreases PARP cleavage in Nutlin-3-treated TC cells compared with control; a representative example of three independent experiments is shown