Figure 3.

PKCη promotes keratinocyte growth arrest in cultured monolayer keratinocytes in high [Ca²⁺] medium. (a) BrdU immunostaining of monolayer-cultured keratinocytes after the medium was changed to one containing high [Ca²⁺] and culture continued for 18 h. Bar, 50 μm. (b) Relative percentages of BrdU-labeled cells in keratinocytes exposed to high [Ca²⁺] medium. Relative % denotes percentages of BrdU-labeled cells in high [Ca²⁺]/low [Ca²⁺] conditions. At least, 1500 cells per sample were counted. Three independent experiments were performed (n=3); ^*P<0.01