Figure 4.

PTE-ET colocalizes with mitochondria in HeLa cells. Cells were incubated with 10 μM phenyltetraene analog of edelfosine (PTE-ET, blue fluorescence) for the indicated times, and the location of mitochondria was examined using MitoTracker (MIT, red fluorescence). Areas of colocalization between PTE-ET and mitochondria in the PTE-ET+MIT panels are purple. The corresponding differential interference contrast (DIC) images are also shown. Images are representative of three independent experiments. Bar, 20 μm