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. 2011 May 26;2(5):e163. doi: 10.1038/cddis.2011.44

Figure 6.

Figure 6

Role of endogenous Gal-1 in regulating epithelial cell survival in vivo following starvation. Wild-type and Lgals1−/− mice were starved during 48 h and intestinal specimens were analyzed. (a) Haematoxylin-eosin staining of representative small bowel longitudinal sections of wild-type and Lgals1−/− mice corresponding to ad libitum fed mice and fasted mice. Magnification × 20 (a and b) and × 10 (c and d). (b) Statistical analysis corresponding to villi length (*P< 0.05). Data are expressed as mean values±S.E.M. (n=10). (c) Statistical analysis corresponding to small bowel weight (*P< 0.05). Data are expressed as mean values±S.E.M. (n=7). (d) Annexin V staining of enterocytes collected from wild-type and Lgals1−/− fasted and ad libitum fed mice. Right: statistical analysis of results obtained with three mice of each group (*P<0.05). (e) Quantitative RT-PCR analysis of mRNA Gal-1 expression in duodenum of wild-type ad libitum fed and fasted mice. Results are expressed as fold increase of mRNA Gal-1 expression, standardized with β-actin mRNA expression (*P<0.05)