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Extinction intensity changes in response to calmodulin conformational changes. (A) Extinction spectra from CutCaM-functionalized LSPR sensor collected in 2 mM CaCl2 (red) and 2 mM EGTA (blue). (B) Magnification of the dashed square region of the spectra shown in 4A, depicting a difference in extinction intensity of 0.002 (A.U.). (C) Time trace showing extinction intensity changes in response to calmodulin conformational transitions.
