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Structural determination of PLA K. pneumoniae CPS. NMR and MS spectra of the fragmented trisaccharide repeat unit after partial acid hydrolysis of purified PLA K. pneumoniae CPS are shown. HSQC (A) and HMBC (B) NMR spectra were used to assign the inter-residue linkages, sequences, and configurations. The MS/MS analysis is shown in C. D and E, chemical structure of PLA K. pneumoniae CPS (D) and after TFA treatment (E). Glc, glucose; GlcA, glucuronic acid; Fuc, fucose; OAc, O-acetyl.
