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. 2011 Apr 26;286(24):21052–21061. doi: 10.1074/jbc.M111.231191

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Effects of NEU4S on cell motility and p38/Hsp27 signaling induced by E-selectin stimulation. a, alteration of cell motility in HT29 cells, with or without E-selectin stimulation. Open bars, mock; gray and black bars, NEU4S transfectants. As a positive control, cells were preincubated with antibodies for sialyl-Le^x and sialyl-Le^a 30 min before seeding onto Boyden chambers (*, p < 0.05; **, p < 0.01). b, phosphorylation of p38 in HT29 cells with E-selectin stimulation. c, phosphorylation of Hsp27 in HT29 (upper) and DLD-1 (lower) cells with E-selectin stimulation. d, actin localization in HT29 cells at 120 min after E-selectin stimulation (left panel, mock; right panel, NEU4S). Cells were fixed and stained with FITC-phalloidin.