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. 2011 Apr 13;286(24):21062–21072. doi: 10.1074/jbc.M110.211409

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — HGF-induced migration requires Sam68 phosphorylation. A, top panel, schematic representation of MAPK consensus (PXX(S/T)P) sites on Myc-tagged WT-Sam68 and phospho-mutant m1 wherein all eight Thr or Ser residues are replaced by Ala (30). Bottom panel, cells were transiently transfected with 2.5 μg of vector and 1 and 2.5 μg of Myc-tagged wild-type Sam68 or Myc-tagged phospho-mutant m1. After 48 h, cells were assayed for HGF-induced migration in Boyden chambers. * denotes significance (p < 0.05) relative to vehicle and between indicated groups, as determined by unpaired Student's t test. B, Western blots showing Myc-tagged Sam68 and endogenous Sam68 expression; C, HGF-induced activation of ERK1/2. Total ERK1/2 served as a loading control.