FIGURE 2.

Secondary assays for latent virus reactivation by AV6. A, the chemical structure of AV6. B, a dose dependence study was performed with AV6. 24STNLSG cells were treated for 48 h in the presence of AV6, at the indicated concentrations in triplicate. The data are expressed as the -fold activation relative to 0.5% DMSO-treated negative control. C, ACH-2 cells were exposed to TNFα (50 ng/ml), VA (1 mm), or AV6 (8 μm) in a 96-well plate for 24 h. Production of HIV-1 Gag p24 protein was measured via ELISA. latent represents an untreated control. Results shown are the means and S.D. of one of two representative experiments. D, the percentage of reactivated 24STNLSG or 19STNLSG cells was evaluated by flow cytometry 48 h after treatment with TNFα (50 ng/ml), VA (1 mm), or AV6 (8 μm).