FIGURE 5.

AV6 cooperates with the HDAC inhibitor VA for enhanced induction of the viral gene expression. 24STNLSG and 19STNLSG cells were treated with VA (1 mm), with AV6 (8 μm) alone, or in combination for 24 h. A, the -fold SEAP induction was calculated as a ratio of relative light units obtained from compound-treated cells versus mock-treated (0.5% DMSO) latent cells. The means and S.D. for the relative light units were obtained from three independent experiments. B, the percentage of GFP-expressing cells was determined via flow cytometry. C, qRT-PCR was performed with RNA isolated from cells 24 h after stimulation with AV6 (8 μm) alone or in combination with VA (1 mm). The indicated regions were analyzed. The results were normalized to the expression of β-actin. The data from three representative experiments are shown.