FIGURE 1.

HuT-102 cells show a Th17-like phenotype and respond to RORC knockdown. A, absolute concentration (ng/ml) of IL-17 in the supernatant collected from HTLV-1-transformed cell culture was determined by ELISA. B, protein expression of RORC, STAT3, and actin in HuT-102 cells was determined by Western blot. C, HuT-102 cells were transfected with siRNAs against RORC and Luc. At 60 h post-transfection, protein expression of RORC, IRF4, STAT3, and PCNA was determined by Western blotting. D, effects of RORC knockdown on the expression of IL-17A, IL-9, and IL-6 were examined by real-time RT-PCR.