TABLE 1.
Expression of genes involved in Th cell differentiation in HTLV-1-transformed T cell lines
Shown is the fold relative to Jurkat cells (*, relative to ED40515(-) cells because Jurkat cells did not express). −−−−, < 0.01; −−−, > 0.01–0.1; −−, > 0.1–0.25; -, > 0.25–0.67; ±, >0.67–1.5; +, > 1.5–4; ++, > 4–10; +++, > 10–100; ++++, > 100.
| Gene | Role in CD4 T cells | Tax negative |
Tax positive |
||
|---|---|---|---|---|---|
| ED40515(-) | MT-1 | HuT-102 | MT-2 | ||
| Th cell cytokines | |||||
| IFN-γ | Main Th1 cytokine | ± | −−−− | − | ++++ |
| IL-4 | Main Th2 cytokine | −−−− | −−−− | + | +++ |
| IL-6 | Induction of Th17 and Treg | −−− | + | ++++ | ++++ |
| IL-9 | Cytokine produced by Th17 and Th9 | − | −−−− | ++++ | ++++ |
| IL-17* | Main Th17 cytokine | ± | −−−− | ++++ | −−−− |
| IL-23 | Induction of Th17 | + | − | + | ± |
| TGF-β1 | Induction of Th17 and Treg | ± | + | −− | −− |
| Transcription factors | |||||
| FoxP3 | Treg master regulator | − | + | − | ++++ |
| GATA3 | Th2 master regulator | −− | − | −− | −− |
| IRF1 | Role in Th1 | − | −− | −− | ± |
| IRF4 | Differentiation of Th2 and Th17 | ++++ | ++++ | ++++ | ++++ |
| RORC | Th17 master regulator | + | — | ++ | −− |
| STAT1 | Differentiation of Th1 | + | + | + | ++ |
| STAT3 | Differentiation of Th17 | + | − | ± | + |
| T-bet | Th1 master regulator | +++ | − | + | +++ |