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pH-dependence of O-acetyl-ADP-ribose hydrolysis by ARH3 and ARH1. Human ARH 3 (1.5 pmol) or ARH1 (375 pmol) was incubated for 5 min with 1 μm OAADPr at the indicated pH as in Fig. 4 before RP-HPLC separation and quantification of ADPr. Blank data without enzyme were subtracted from each of these points. The experiment was repeated three times with similar results.
