FIGURE 5.

Physiological consequences of TR4 acetylation. A, TR4 acetylation mutant blocks ARA55 effect on the mRNA expression levels of CD36, a target gene of TR4. Hep1-6 cells were cotransfected with pCDNA3-flagTR4 and pCDNA4-flagARA55 as indicated. After 24 h, the cells were harvested, and CD36 mRNA levels were examined by real time RT-PCR. The internal control was 18 S rRNA, and the mRNA expression in control cells (with empty vector) was set as 1. The data represent the means ± S.D. of triplicate samples. B, TR4 acetylation mutant blocks ARA55 effect on the protein expression levels of CD36, a target gene of TR4. Hep1-6 cells were cotransfected with pCDNA3flagTR4 and pCDNA4-flagARA55 as indicated. After 48 h, the cells were harvested, and protein levels of CD36 as well as TR4 and ARA55 were examined by Western blot analysis. GAPDH served as a loading control.