FIGURE 5.

TGM2 contributes to acquired TRAIL resistance. A–C, the indicated cells were pretreated with cystamine (CTM; 1 mm) for 1 h or were left untreated, followed by exposure to TRAIL (150 ng/ml for A549 and H1568, 100 ng/ml for H460) for 30 h. Cell death was detected by lactate dehydrogenase leakage assay (cytotoxicity assay). Data shown are mean ± S.D. **, p < 0.01. D, A549-TR cells were transfected with 20 nm of TGM 2 siRNA or NC siRNA. Forty-eight hours after transfection, the cells were treated with TRAIL (150 ng/ml) for 30 h or were left untreated. Cell death was detected as described in A.