FIGURE 1.

4PBA increases ERp29 mRNA expression in IB3-1 cells. A, ERp29 transcript was quantitated using a ribonuclease protection assay in IB3-1 cells treated for the indicated times with 1 mm 4PBA as described under “Experimental Procedures.” The hybridization of 18 S rRNA as an internal standard was constant under these conditions. These data are representative of n = 8 independent experiments. B, relative amount of ERp29 mRNA compared with the zero time point was determined by densitometry (n = 8 independent sample sets, each normalized by 18 S rRNA content). Means ± S.E. are shown with significance determined by one-way ANOVA in comparison with t = 0.