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GP Ibβ/GP IX resides in the same density fractions as the GP Ib-IX complex in a cholesterol-dependent manner. A, CHO cells expressing only Ibβ and IX polypeptides (CHOβIX) were lysed with 0.5% Brij 35 or 0.1% Triton X-100 followed by a sucrose density gradient fractionation. B, GP Ibβ and GP IX resided in similar fractions as the GP Ib-IX complex in CHOαβIX cells (Fig. 2B), which is also sensitive to cholesterol deprivation.
