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. 2011 Apr 15;286(24):21330–21339. doi: 10.1074/jbc.M110.202424

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Overexpression of LPCAT1 or LPCAT2 increases LD-associated LPCAT activity. A, C, and E, normalized LPCAT activity on LDs isolated from oleate-treated COS7, A431, and HuH7 cells, transfected with either empty vector (Control), LPCAT1-overexpression vector (LPCAT1), or LPCAT2-overexpression vector (LPCAT2). Data are mean ± S.D. from triplicate determinations of four independent experiments. Significance was obtained by unpaired t test analysis. B, D, and F, Western blot against LPCAT1, LPCAT2, and GAPDH (loading control) in the lysates.