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. 2011 Apr 15;286(24):21330–21339. doi: 10.1074/jbc.M110.202424

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Knockdown of LPCAT1 and LPCAT2 decreases LD-associated LPCAT activity. A and C, Western blots against LPCAT1, LPCAT2, and GAPDH (loading control) in cell lysates from oleate-treated A431 and HuH7 cells, transfected with either nontargeting siRNA (Control), or siRNA targeting LPCAT1 (L1-A/B/C) and LPCAT2 (L2-A/B) (A and B) or siRNA targeting LPCAT1 only (C and D). B and D, normalized LPCAT activity on LDs isolated from oleate-treated A431 and HuH7 cells, transfected as described in A and C. Data are mean ± S.D. from triplicate determinations of four independent experiments. Significance was obtained by unpaired t test analysis. E, mean area of LDs from HuH7 cells, treated with siRNA as indicated (no oleate supplementation), was quantified from fluorescence microscopy images. Each bar contains data from at least 12 images with an average of 10 cells per image (***, p ≤ 0.001).