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. 2011 Apr 15;286(24):21330–21339. doi: 10.1074/jbc.M110.202424

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — LDs can locally synthesize PC by the Lands cycle but not by the Kennedy pathway. Lysates of A431 cells, cultured in the presence of 100 μm oleic acid, were subjected to floatation in a sucrose density gradient. Samples of the LD fraction (LD), the floating membrane fraction + bottom fraction (membranes), and the total lysates (lysate) were incubated in the presence of either [¹⁴C]CDP-choline and DAG (left panel, CPT assay) or of [³H]acyl-CoA and LPC (right panel, LPCAT assay). Lipids were extracted and analyzed by TLC followed by autoradiography. Radioactive spots were identified by co-migrating standards. PC, phosphatidylcholine; FFA, free fatty acid.