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. 2011 Apr 22;286(24):21340–21352. doi: 10.1074/jbc.M110.175042

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — A and B, NP-HPLC profiles of 2AB-labeled N-glycans released from the sugar moieties of the 66-kDa glycoprotein after PNGase F (A) and Endo H digestion (B) are shown. Retention times were converted to a GU scale relative to a dextran ladder. GU values are shown beneath the designation of each. C–E, mass determination of PNGase F-released glycans from the 66-kDa glycoprotein is shown. Positive ion MALDI-TOF MS of the total 2AB-labeled N-glycans (C) and unlabeled N-glycans (D) released from the 66-kDa glycoprotein by PNGase F is shown. E, negative ion ESI mass spectra of the total N-glycans released from the 66-kDa glycoprotein by PNGase F are shown. Interpretation of peak masses in terms of sugar composition and the calculated masses of the ions are summarized in Table 1.