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Negative ion ESI-collision-induced dissociation (phosphate adducts) of N-linked glycans released from 66-kDa glycoprotein by PNGase F. Shown are compound 1 at m/z 1991 ([1F+H2PO4]− ion) (A), compound 3 at m/z 2153 ([4F+H2PO4]− ion) (B), compound 2 at m/z 2123 ([3F+H2PO4]− ion) (C), and compound 4 at m/z 2286 ([1F+H2PO4]− ion) (D). Ion nomenclature follows the method of Domon and Costello (47).
