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Up-regulation of Adam12 by Notch is not cell-specific. NIH3T3, NMuMG, or C2C12 cells were infected with caNotch1 or control retroviruses. After 24 or 48 h, the level of Adam12 mRNA was evaluated by semiquantitative RT-PCR; Gapdh is an internal control. The experiment was repeated twice, with similar results.
