FIGURE 8.

caNotch1 specifically increases the level of ADAM12L splice variant mRNA. A, the diagram of mouse Adam12 transcript and two alternatively spliced human ADAM12 mRNAs, ADAM12L and ADAM12S. The two human variants have the common 5′-UTRs and most of the coding region (black boxes) and divergent sequences in the C-terminal end of the coding region (gray and hatched boxes) and in the 3′-UTRs (gray and hatched lines). B, HEK293 and MCF7 cells were infected with caNotch1 or control retroviruses, and the levels of ADAM12L and ADAM12S were examined by semiquantitative RT-PCR 48 h after infection, using splice variant-specific primer sets; β-ACTIN indicates an internal control. C and D, the intensities of ADAM12L and ADAM12S bands shown in panel B were quantified by densitometry using the ImageJ software. The -fold changes in ADAM12 mRNA, normalized to β-actin, in HEK293 cells (C) and MCF7 cells (D) are shown. The data represent the means ± S.E. from 3 independent experiments. *, p < 0.05, n.s., not significant.