Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Apr 4;286(24):21535–21545. doi: 10.1074/jbc.M111.221143

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 3. — Mono Q anion exchange chromatography of polySia-NCAM-Fc synthesized by each STX/PST pair. polySia-NCAM-Fc secreted from CHO cells stably expressing NCAM-Fc and each of STX(WT), STX(G421A), or STX(C621G) in the presence of the PST(WT) was collected from culture medium using a protein G-Sepharose column and subjected to Mono Q anion exchange chromatography. The NaCl gradient is indicated in the graph by the dotted line. The eluate was collected and dot-blotted onto PVDF membrane, followed by immunostaining with anti-polySia (12E3) antibody. The polySia staining exhibited by each sample prior to anion exchange chromatography was set equal to 1.0. The 12E3-relative activity shows the amounts of polySia in each fraction. ■, polySia-NCAM-Fc derived from STX(WT); ●, polySia-NCAM-Fc derived from STX(G421A); ▴, polySia-NCAM-Fc derived from STX(C621G). The relationship between NC and the NaCl concentration at which polySia-NCAM-Fc was eluted is indicated in the figure. The relationship with log(NC) and NaCl concentration was already confirmed (28).